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Promega Corporation

Sequencing Grade Modified Trypsin, Frozen, Product Information

  • Instructions for Use of Product(s)
  • V5113

Literature # 9PIV5113

Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing of HPLC peptide analysis. In addition, auto-proteolysis can result in the generation of pseudotrypsin, which has been shown to exhibit chymotrypsin-like specificity. Promega Sequencing Grade Modified Trypsin is porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion. In enzymatic stability tests, modified trypsin was found to retain greater than two times the activity of unmodified trypsin.

Printed in USA. Revised 3/13.


PDF Icon Complete Protocol PDF (149kb)

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