Literature # 9PIM210
Tth DNA Polymerase is a thermostable enzyme that replicates DNA at 74°C and exhibits a half-life of 20 minutes at 95°C. Tth catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and the polymerization of nucleotides into DNA using an RNA template in the 5´→3´ direction in the presence of manganese. The enzyme has a molecular weight of 94,000 daltons as estimated from the predicted amino acid sequence and exhibits 5´→3´ exonuclease activity. Tth is recommended for use in PCR, RT-PCR, reverse transcription and primer extension reactions at elevated temperature.
Printed in USA. Revised 9/11.