We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

pGL4.52[luc2P/STAT5RE/Hygro] Vector Protocol

  • Instructions for Use of Product(s)
  • E4651

Literature # 9PIE465

The pGL4.52[luc2P/STAT5 RE/Hygro] Vector contains five copies of a STAT5 response element (STAT5 RE) that drives transcription of the luciferase reporter gene [luc2P (Photinus pyralis). luc2P is a synthetically derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P gene contains hPEST, a protein destabilization sequence, which allows luc2P protein levels to respond more quickly than those of luc2 to induction of transcription. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a gene for hygromycin resistance to allow selection of stably transfected mammalian cell lines.

Printed in USA 7/12.

 

PDF Icon Complete Protocol PDF (218kb)

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.