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Promega Corporation

pGL4.29[luc2P/CRE/Hygro] Vector Protocol

  • Instructions for Use of Product(s)
  • E8471

Literature # 9PIE847

The pGL4.29[luc2P/CRE/Hygro] Vector contains a cAMP response element (CRE) that drives the transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance.

Printed in USA. Revised 6/13.

 

PDF Icon Complete Protocol PDF (119kb)

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