Instructions for Use of Product(s)
Literature # 9PIC852
The pFN6K (HQ) Flexi® Vector is designed for use with the Flexi® System, Entry/Transfer (Cat.# C8640), and the Flexi® System, Transfer (Cat.# C8820). The vector contains a T7 promoter for bacterial or in vitro expression of a protein-coding region. The vector appends an N-terminal MKHQHQHQAIA coding region, which can be used to purify the expressed protein using the MagneHis™, MagZ™, HisLink™ 96 or HisLink™ Protein Purification Systems (Cat.# V8500 and V8550; V8830; V3680 and V3681; and V8821, respectively). The vector contains the lethal barnase gene for positive selection of the insert, a kanamycin-resistance gene for selection of the plasmid and unique SgfI and PmeI sites, which allow easy insertion or transfer of the sequence of interest. Inserts containing a protein-coding region can be easily transferred from the pFN6K (HQ) Flexi® Vector to other Flexi® Vectors with different expression options.
Summary of Changes
The following change was made to the 12/14 revision of this document:
1. Expired patent or license statements were removed.
Printed in USA. Revised 12/14.