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Promega Corporation

pFN6K (HQ) Flexi® Vector Product Information

  • Instructions for Use of Product(s)
  • C8521

Literature # 9PIC852

The pFN6K (HQ) Flexi® Vector is designed for use with the Flexi® System, Entry/Transfer (Cat.# C8640), and the Flexi® System, Transfer (Cat.# C8820). The vector contains a T7 promoter for bacterial or in vitro expression of a protein-coding region. The vector appends an N-terminal MKHQHQHQAIA coding region, which can be used to purify the expressed protein using the MagneHis™, MagZ™, HisLink™ 96 or HisLink™ Protein Purification Systems (Cat.# V8500 and V8550; V8830; V3680 and V3681; and V8821, respectively). The vector contains the lethal barnase gene for positive selection of the insert, a kanamycin-resistance gene for selection of the plasmid and unique SgfI and PmeI sites, which allow easy insertion or transfer of the sequence of interest. Inserts containing a protein-coding region can be easily transferred from the pFN6K (HQ) Flexi® Vector to other Flexi® Vectors with different expression options.

Printed in USA. Revised 7/13.

 

PDF Icon Complete Protocol PDF (198kb)

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