The wheat germ cell-free TnT® SP6 High-Yield Protein Expression System produces high levels of functional protein. Functional protein yield can reach 100µg/ml in batch mode and 400–500µg/ml in dialysis mode. A method for the production of functional procaspase-3 and the mature protein, caspase-3, using the same vector with different modes of protein synthesis is described. Full-length procaspase-3 was produced (>70µg/ml) in batch mode, while functional processed caspase-3 was produced (~400–500µg/ml) in dialysis mode.
Promega Notes 94, 31–35.
Kate Qin Zhao, Robin Hurst, Sarah Wheeler, Becky Godat, Jami English, Jim Hartnett and Michael Slater