This article introduces the PureYield™ Plasmid Midiprep System. The system is designed to rapidly isolate highly pure plasmid DNA for transfection, in vitro expression, automated fluorescent DNA sequencing and other applications. The unique design of the new PureYield™ columns yields 100–200µg of plasmid DNA in less than 30 minutes and elutes in a concentrated volume, eliminating the need to ethanol precipitate plasmid DNA prior to use. An Alternative Lysate Clearing Protocol can extend the system to purify up to 400µg of plasmid DNA. The PureYield™ System can save up to three-and-a-half hours compared to other plasmid midiprep methods, freeing researchers to focus on more challenging aspects of their work.
Promega Notes 88, 5–8.
Doug White, Cheryl Bailey, Hemanth Shenoi and Rex Bitner