This report demonstrates use of the FastBreak™ Cell Lysis Reagent and the MagneHis™ Protein Purification System to purify His-tagged proteins from insect and mammalian cell lysates and culture media in the presence or absence of serum. The FastBreak™ Reagent, 10X, is added directly to cells in culture, achieving gentle, efficient lysis in 15 minutes without the need for centrifugation or mechanical disruption. Lysis can also be achieved by adding the FastBreak™ Reagent directly to attached or pelleted cells at a 1X concentration. The MagneHis™ Protein Purification System can then be used to purify His-tagged proteins present in the lysate or in culture media. Thus, the combination of the FastBreak™ Reagent and the MagneHis™ Protein Purification System allows efficient purification of His-tagged proteins from diverse sample types.
Promega Notes 87, 29–32.