DNA analysis techniques have become central in many aspects of modern basic and clinical research. These methods require high-quality DNA from diverse sample types that might include blood, tissue, buccal swabs, body fluids and feces. Traditionally, smaller laboratories have used time- and labor-intensive manual genomic DNA purification methods, as automated instruments have been too large, too expensive and not suited for their lower throughput. This article describes a new system for 1-to 15-sample, low- to moderate-throughput automated genomic DNA purification. The MagneSil® KF, Genomic System is designed for use on the Thermo Electron KingFisher® mL. Using this instrument, DNA purification takes under 25 minutes. The purified DNA is suitable for PCR, fluorescent STR, as well as more stringent applications such as multiplex PCR (e.g., Promega Y Chromosome Deletion Detection System), and SNP detection systems.
Promega Notes 85, 21–24.
Rex Bitner1, Susan Koller1, Hemanth Shenoi1, Kenneth S. Henderson2, Paulo Silva2 and Gregory J. Tsongalis, 3
1Promega Corporation, 2Charles River Laboratories, Wilmington, MA, 3Hartford Hospital, Hartford, CT