A new synthetic gene encoding Renilla luciferase has been designed to provide greater expression efficiency and reliability as a genetic reporter. The synthetic Renilla gene (hRluc) is codon optimized for mammalian cells and is designed to minimize unintended mechanisms of genetic regulation. The gene is provided in seven different vector configurations to support applications both as a control reporter and a primary reporter. These vectors can be used either with the Dual-Luciferase® Reporter Assay System or the newly developed Renilla Luciferase Reporter Assay System. The new Renilla Luciferase Reporter Assay System provides high sensitivity due to a novel formulation that virtually eliminates background luminescence common to conventional assays. This new reagent provides rapid and linear quantitation over eight logs of enzyme concentration.
Promega Notes 79, 6–11.
Yao Zhuang, Braeden Butler, Erika Hawkins, Aileen Paguio, Laurie Orr, Monika Wood and Keith V. Wood