Design of Homogeneous Luciferase Assays

Homogeneous luciferase assays can provide quantitative analysis of luciferase expression with more speed and less effort than traditional luciferase assays. However, the design of homogeneous assays can substantially affect data quality and quantitative robustness. Of greatest concern is achieving uniform luminescence potential within the sample wells. This can be compromised by concentration-dependence of the assay reagent and by formation of luminescence gradients when the reagent is combined with cell culture media. The formulation of the Promega Steady-Glo® Reagent contains innovative features that overcome these limitations.

Promega Notes 74, 3–6.