The pCI-neo Mammalian Expression Vector contains a cytomegalovirus (CMV) immediate-early enhancer/promoter, an optimized chimeric intron and the simian virus 40 (SV40) late polyadenylation signal. These three elements combine to yield strong, constitutive expression of the cloned genes in mammalian cells. The vector also contains a neomycin phosphotransferase gene for selection of stably transfected clones, a T7 promoter, an f1 origin of replication, unique restriction enzyme sites flanking each of the elements of the vector, a high-copy plasmid replicon, and a versatile multiple cloning region. In this article, we compare the expression of three transgenes cloned into the Promega pCI-neo Vector and the pcDNA4/HisMax Vector from Invitrogen.
Promega Notes 75, 33–35.
Brian D. Almond and Elaine T. Schenborn