Promega's new SV Total RNA Isolation System offers a complete system for the isolation of quality RNA from small amounts of starting material. This system provides a fast and simple technique for the preparation of purified and intact total RNA from tissues, cultured cells and whole blood samples. This purification is achieved without the use of phenol/chloroform extractions and employs a simple DNase step to eliminate genomic DNA efficiently. No genomic DNA contamination is seen when the RNA is analyzed by reverse transcription-PCR (RT-PCR). In this article, we demonstrate that RNA isolated using this system is highly pure and is ideally suitable for applications such as RT-PCR.
Promega Notes 64, 7.
Paula Brisco, Jacqui Sankbeil and Dan Kephart