The TnT® T7 Quick Coupled Transcription/Translation System offers researchers a convenient new alternative for eukaryotic in vitro translation -- a one tube, coupled transcription/translation reaction. This new addition to the TnT® Coupled Transcription/Translation Systems simplifies the process by including all of the reaction components in a single TnT® T7 Quick Master Mix. To use this system, 0.2–2.0µg of either circular or linear DNA is added to an aliquot of the Master Mix and incubated in a 50µl reaction volume for 60–90 minutes at 30°C. Synthesized proteins are then analyzed by SDS-PAGE and autoradiography or by functional activity.
Promega Notes 58, 8.
Robin Hurst, Mark Maffitt, Elizabeth Murray, James Kappelman, Qin-yu Xu, Jacqui Mendez, Braeden Butler, Angela Ryan and Gregory S. Beckler