We developed a cell-based assay for endogenous calpain activity using a cell-permeant
luminescent calpain substrate. Using the protocol described here, we were able
to identify potent, reproducible inhibitors of calpain activity in a
high-throughput screening application.
Cell Notes 18, 6–8.
Katheleen Seyb1,2, Jake Ni1, Mickey Huang1, Eli Schuman1, Mary L. Michaelis2 and Marcie A. Glicksman1