Immunohistochemical Staining Using Promega AntiACTIVE® and Apoptosis Antibodies

This article describes protocols for tissue staining with Promega polyclonal antibodies in mouse and rat systems. Protocols are provided for Anti-ACTIVE^® MAPK (Cat.# V8031), Anti-ACTIVE^® JNK (Cat.# V7931), Anti-ACTIVE^® p38 (Cat.# V1211), and Anti-ACTIVE^® CaM KII pAbs (Cat.# V1111). In addition Promega protocols using polyclonal antibodies useful for the study of apoptosis (Anti-PARP p85 Fragment pAb [Cat.# G7341], Anti-ACTIVE^® Caspase-3 pAb [Cat.# G7481], and Anti-pS⁴⁷³ Akt pAb [Cat.# G7441]) are described. Immunohistochemical data are shown for each of the above antibodies.

Cell Notes 1, 4–7.