Promega Corporation

Doing Good Science: Authenticating Cell Line Identity

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Abstract

Much of biomedical research—medicine, genetics, drug discovery, vaccine development, reconstructive medicine, basic science, HIV testing/treatment, and cell biology—is done with cultured cells obtained from major repositories or fellow researchers. An estimated 15–20% of the time, cells used in experiments have been misidentified or cross-contaminated with another cell line. Although major repositories now authenticate cell line identity, many are calling for all researchers to test and authenticate cell line identity using standard genotyping techniques like STR analysis.

Cell Notes 22, 15–17.

Jill Harley Dunham1 and Pam Guthmiller2

1Emory University, Department of Pharmacology, and 2Promega Corporation
2008

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