This guide lists a set of experiments that can be used or adapted for use by laboratories performing an internal validation of the Plexor® HY System. These studies are intended to show that this quantitation method is a sensitive and reliable method to estimate the quantity of DNA recovered upon extraction from a sample. In addition, these experiments will determine: 1) the optimal DNA concentration for input into short tandem repeat (STR) amplification reactions and 2) when it is more appropriate to perform Y-STR analysis rather than autosomal STR analysis for mixtures of male and female DNA.
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