Turn ideas into assays FASTER!
Develop your own assays quickly and easily with the READIT® System.
Design assays for SNPs, insertions, deletions and chromosomal translocations.
Genotyping is straightforward using the READIT® System which is based
on the hybridization of user designed interrogation probes to PCR products.
Perfect hybridization of the 3´-end of an allele-specific probe leads
to the generation of light from luciferase through coupled enzymatic reactions.
A homozygous sample will generate light from only one of the allele-specific interrogation
probes, where a heterozygous sample will generate light from both probes.
Simple assay design — Quick turn-around time
Probe design made simple. Design interrogation probes in one step with the
READIT® Online Probe Design software. Using the online program, simply
cut and paste or type in a DNA sequence, indicate the position of the genetic
variation and the program will recommend optimal probe designs. The Probe
Design Program incorporates the knowledge and experience of Promega scientists
to develop successful READIT® assays. Problematic DNA sequences like
GC-rich regions can be addressed.
Strategy for analysis of the bcr-abl chromosomal translocation
using the READIT® Assay. Multiple primers and probes can be designed
to identify the known genetic variation.
Interrogation Probes are PCR-grade oligos, typically 20-24 bases in length.
They can be ordered from any supplier; no modified bases or special conjugated
tags, enzymes or labels are required.
All READIT® reactions are performed under identical conditions so minimal
optimization is required to move from assay to assay or target to target.
Multiple assays can be mixed on one plate.
The READIT® System is an effective tool for pharmacogenetics, as well
as characterizing genetic disease and the agronomic properties of plants.
Accuracy | Flexible
Throughput | Assay Design Versatility
|
