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READIT® Interrogation Probe Design Program for Allele Difference Analysis

To discuss an evaluation of the system, e-mail techserv@promega.com or view our worldwide contact information page.

   
 

Introduction to the READIT® SNP Genotyping System: Assay Design 

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To start a new assay using the READIT® SNP Genotyping System, you must first know some information about your target. Once you know your target, you can design READIT® Interrogation Probes to differentiate one allele from the other. After you know you know the design of your READIT® Interrogation probes, you can design the target amplification including which primer to protect for the production of a target for the READIT® Reaction.