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Consider the READIT® SNP
Genotyping System
Genotyping for clinical diagnostics or research applications requires accuracy, flexible throughput and versatile assay design. The READIT® SNP Genotyping System is your solution. The first consideration is getting an accurate result. The READIT® assay combines enzyme fidelity, stringent probe hybridization and statistical controls to ensure that you get the right call, providing unsurpassed confidence in the data generated by your experiment. Validation studies have shown that the READIT® System has a genotyping accuracy of
99.9%
The READIT® System allows you to interrogate PCR products to determine the presence of a known sequence, detect SNPs, estimate allelic frequency, detect chromosomal translocations and perform allele correlation studies.
(view a presentation on applications)
You design the interrogation probes using our web based probe design software
(view a presentation on assay design). Synthesize the unmodified PCR grade probes or purchase them from the oligo supplier of your choice and you are ready to start genotyping.
Who can use the READIT®
SNP
Genotyping System
Whether you are a small lab with hundreds of assays or a big lab with hundreds of thousands, the
READIT® technology can meet your throughput requirements. Generate one hundred genotypes in 90 minutes with a multi-channel pipette and a 96-well plate luminometer or 384 genotypes in the same time with a liquid handler and a 384 well luminometer. Choose what suits your lab and your budget.
What are the costs of using the READIT®
SNP
Genotyping System?
The READIT® technology is the most cost effective way to generate genotype data. Small labs can implement the system with low cost, readily available instrumentation, while high throughput labs can work with liquid handler robots to genotype thousands of samples a day in 384 well format for under $1 a call.
The READIT® Assay uses optimized reagents, formatted to minimize pipetting, ease automation and ensure robustness. A quick enzymatic clean-up of the PCR product is followed by denaturation and probe hybrization at
55oC. All the assays use the same conditions allowing you to run multiple assays at one time and making new assay design a snap. The READIT® Master Mix detects perfect probe matches and produces light from luciferase bioluminescence detection of ATP generated.
(view a presentation on biochemistry READIT® reaction)
Data Management and the READIT®
SNP
Genotyping System
A luminometer measures the light output and the READIT® Calculator automatically makes the genotype call. The genotype information is saved to a database available for further analysis.
(view a presentation on READIT® data analysis and management)
If you are ready to start genotyping, contact us to arrange an evaluation of the READIT®
Technology.
readit@promega.com
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