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The T7 RiboMAX™ Express RNAi System is an in vitro transcription system designed for producing milligram amounts of double-stranded RNA (dsRNA) in a short amount of time. The dsRNA is free of protein and other contaminants and is suitable for use in RNA interference (RNAi) in both mammalian and nonmammalian systems.
The T7 RiboMAX™ Express RNAi System can be used to synthesize short interfering RNAs (siRNAs) of 21bp for use in mammalian systems. siRNAs synthesized in vitro have been demonstrated to be as effective as chemically synthesized siRNAs for inducing RNAi in mammalian cells.
In addition, the T...
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In addition, the T7 RiboMAX™ Express RNAi System can be used for the synthesis of dsRNA molecules of approximately 200bp or greater, which can be applied to nonmammalian systems. Two complementary RNA strands are synthesized from DNA template (either plasmid or PCR product). The resulting RNA strands are annealed after the transcription reaction to form dsRNA. Any remaining single-stranded RNA and DNA template are removed with a nuclease digestion step. The dsRNA is then purified by isopropanol precipitation and can be introduced into the organism of choice for RNAi applications.
For more information, see the Protocols & Applications Guide.
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T7 RiboMAX™ Express RNAi System
RNase A Solution
Sodium Acetate, 3.0M (pH 5.2)
Enzyme Mix, T7 Express
RiboMAX™ Express T7 2X Buffer
pGEM® Express Positive Control Template
RQ1 RNase-Free DNase
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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Store all components at –20°C, except RNase A, which should be stored at 22–25°C after the initial thaw.
For product intended use please see Patents & Disclaimers tab.
P1700 For Research Use Only. Not for Use in Diagnostic Procedures.
P1700 This product is covered under license from Carnegie Institution of Washington under U.S. Pat. Nos. 6,506,559, 7,538,095, 7,560,438, Australian Pat. No. 743798 and other patents pending. Commercial use of this product will require a separate license from Carnegie.
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