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Promega Corporation

GeneClip™ U1 Hairpin Cloning Systems

The GeneClip™ U1 Hairpin Cloning Systems consist of linearized plasmids designed for fast and easy cloning of human target sequences to express short hairpin RNAs (shRNAs) in human cells. After transfection into human cells, in vivo expression of short interfering RNAs (siRNAs) can be effectively achieved from DNA constructs that contain a U1 RNA polymerase promoter and a siRNA template. The U1 promoter has been used successfully to generate hairpin siRNAs in vivo.

To insert hairpin siRNAs into the pGeneClip™ Vectors, two short DNA oligonucleotides are annealed to form a DNA insert that contains the hairpin ...

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  • Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart

GeneClip™ U1 Hairpin Cloning System-Basic

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GeneClip™ U1 Hairpin Cloning System-Basic

 Components

  • 2X Rapid Ligation Buffer

    C671A1 x 200μl
  • Oligo Annealing Buffer

    C838A1 x 1ml
  • pGeneClip™ Basic Vector

    C875A1 x 1.2μg
  • T4 DNA Ligase

    M180A1 x 100u
  • Nuclease-Free Water

    P119A1 x 1,250μl
1 system
- C8750 $ 386.00 Add to cart

GeneClip™ U1 Hairpin Cloning System-Puromycin

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GeneClip™ U1 Hairpin Cloning System-Puromycin

 Components

  • 2X Rapid Ligation Buffer

    C671A1 x 200μl
  • Oligo Annealing Buffer

    C838A1 x 1ml
  • pGeneClip™ Puromycin Vector

    C876A1 x 1.2μg
  • T4 DNA Ligase

    M180A1 x 100u
  • Nuclease-Free Water

    P119A1 x 1,250μl
1 system
- C8760 $ 386.00 Add to cart

GeneClip™ U1 Hairpin Cloning System-Hygromycin

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GeneClip™ U1 Hairpin Cloning System-Hygromycin

 Components

  • 2X Rapid Ligation Buffer

    C671A1 x 200μl
  • Oligo Annealing Buffer

    C838A1 x 1ml
  • pGeneClip™ Hygromycin Vector

    C877A1 x 1.2μg
  • T4 DNA Ligase

    M180A1 x 100u
  • Nuclease-Free Water

    P119A1 x 1,250μl
1 system
- C8770 $ 386.00 Add to cart

GeneClip™ U1 Hairpin Cloning System-Neomycin

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GeneClip™ U1 Hairpin Cloning System-Neomycin

 Components

  • 2X Rapid Ligation Buffer

    C671A1 x 200μl
  • Oligo Annealing Buffer

    C838A1 x 1ml
  • pGeneClip™ Neomycin Vector

    C878A1 x 1.2μg
  • T4 DNA Ligase

    M180A1 x 100u
  • Nuclease-Free Water

    P119A1 x 1,250μl
1 system
- C8780 $ 386.00 Add to cart

GeneClip™ U1 Hairpin Cloning System-hMGFP

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GeneClip™ U1 Hairpin Cloning System-hMGFP

 Components

  • 2X Rapid Ligation Buffer

    C671A1 x 200μl
  • Oligo Annealing Buffer

    C838A1 x 1ml
  • pGeneClip™ hMGFP Vector

    C879A1 x 1.2μg
  • T4 DNA Ligase

    M180A1 x 100u
  • Nuclease-Free Water

    P119A1 x 1,250μl
1 system
- C8790 $ 430.00 Add to cart


GenBank®/EMBL Accession Number

pGeneClip™ Basic Accession Number AY744385; pGeneClip™ Basic Vector sequence text file.
pGeneClip™ Puromycin Accession Number AY745747; pGeneClip™ Puromycin Vector sequence text file.
pGeneClip™ Hygromycin Accession Number AY745745; pGeneClip™ Hygromycin Vector sequence text file.
pGeneClip™ Neomycin Accession Number AY745746; pGeneClip™ Neomycin Vector sequence text file.
pGeneClip™ hMGFP Accession Number AY744386; pGeneClip™ hMGFP Vector sequence text file.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

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