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Primer Extension System—AMV Reverse Transcriptase can be used to quantitate specific mRNA transcripts and map the start sites of transcription. An end-labeled oligonucleotide is hybridized to RNA and is used as a primer by reverse transcriptase in the presence of deoxynucleotides. The RNA is thus reverse transcribed into cDNA and is analyzed on a denaturing polyacrylamide gel. The length of the cDNA reflects the number of bases between the labeled nucleotide of the primer and the 5´-end of the RNA; the quantity of cDNA product is related to the amount of targeted RNA.
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Primer Extension System-AMV Reverse Transcriptase
1.2kb Kanamycin Positive Control RNA
Primer Extension 2X Buffer
Primer Extension Sodium Pyrophosphate
ΦX174 DNA/HinfI Dephosphorylated Markers
Primer Extension Control Primer
AMV Reverse Transcriptase
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase 10X Buffer
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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All components must be stored at –20°C, except for the control RNA, which must be stored at –70°C.
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E3030 For Research Use Only. Not for Use in Diagnostic Procedures.
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