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Promega Corporation

Primer Extension System—AMV Reverse Transcriptase

Primer Extension System—AMV Reverse Transcriptase can be used to quantitate specific mRNA transcripts and map the start sites of transcription. An end-labeled oligonucleotide is hybridized to RNA and is used as a primer by reverse transcriptase in the presence of deoxynucleotides. The RNA is thus reverse transcribed into cDNA and is analyzed on a denaturing polyacrylamide gel. The length of the cDNA reflects the number of bases between the labeled nucleotide of the primer and the 5´-end of the RNA; the quantity of cDNA product is related to the amount of targeted RNA.

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Product Size Conc. Catalog # *List Price QTY Add to Cart

Primer Extension System-AMV Reverse Transcriptase

40 reactions
- E3030 $ 183.00 Add to cart


Storage Conditions

All components must be stored at –20°C, except for the control RNA, which must be stored at –70°C.

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Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

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