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Promega Corporation

HeLaScribe® Nuclear Extract in vitro Transcription System

The most well characterized cell-free system for in vitro transcription of eukaryotic genes is derived from HeLa cell nuclei. HeLa nuclear extracts can support accurate transcription initiation by RNA polymerase II and exhibit both basal and regulated patterns of RNA polymerase transcription. The nuclear extract is also a source for a variety of transcription factors, DNA-binding proteins and the enzymatic machinery involved in RNA processing.

The HeLa Nuclear Extract included in the HeLaScribe® Nuclear Extract in vitro Transcription System is prepared by a modification of the method of Dignam et al. Extracts p...

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HeLaScribe® Nuclear Extract in vitro Transcription System

40 reactions
- E3110 $ 381.00 Add to cart


Storage Conditions

Store at –70°C. Avoid multiple freeze-thaw cycles of the extract.

Unit Definition

One unit is defined as the amount of HeLaScribe® Extract required for the incorporation of 50fmol of nucleotides into a 363-nucleotide run-off transcript generated from the CMV immediate early promoter fragment per hour at 30°C using standard conditions.

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