T7 RiboMAX™ Express Large Scale RNA Production System

The T7 RiboMAX™ Express Large Scale RNA Production System is an in vitro transcription system designed for the consistent production of milligram amounts of RNA in a short amount of time. Due to optimization of the enzyme mix and transcription buffer, yields of 5–8.5mg/ml are generated in 30 minutes, compared to 2–4 hours with other commercially available systems. To minimize pipetting steps and errors, the 2X transcription buffer includes all four rNTPs. In addition, the system includes RQ1 RNase-Free DNase for the removal of plasmid template after transcription.

Due to the combined 2X buffer and rNTPs, the ...

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The T7 RiboMAX™ Express Large Scale RNA Production System is an in vitro transcription system designed for the consistent production of milligram amounts of RNA in a short amount of time. Due to optimization of the enzyme mix and transcription buffer, yields of 5–8.5mg/ml are generated in 30 minutes, compared to 2–4 hours with other commercially available systems. To minimize pipetting steps and errors, the 2X transcription buffer includes all four rNTPs. In addition, the system includes RQ1 RNase-Free DNase for the removal of plasmid template after transcription.

Due to the combined 2X buffer and rNTPs, the T7 RiboMAX™ Express System is not recommended for the synthesis of RNA for applications that require capped RNA. For synthesis of capped RNA, please order the standard RiboMAX™ Large Scale RNA Production System—T7 (Cat.# P1300).

Features - Benefits

• Fast: The T7 RiboMAX™ Express System produces milligram amounts of RNA in as little as 30 minutes rather than 2–4 hours as with other commercially available systems.
• Convenient: The four rNTPs and 2X transcription buffer have been combined, thus minimizing pipetting errors and setup time.
• Flexible: Efficiently transcribes DNA templates of varying sizes. Works with transcripts as short as 21bp.

Applications

• Generation of large quantities of uncapped transcripts for in vitro or in vivo translation.
• In vitro synthesis of tRNA, rRNA, RNA virus genomes and ribozymes.
• Production of substrates for RNA splicing, RNA secondary structure, antisense RNA and RNA:protein interaction studies.
• Synthesis of long RNAs for nonmammalian RNA interference.

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