ChipShot™ Labeling and Clean-Up Systems

The ChipShot™ Labeling and Clean-Up Systems enable researchers to generate consistent, reproducible and sensitive microarray data. The ChipShot™ Systems make it easy for individual researchers to perform RNA and microarray manipulation, regardless of their experience. The protocols are simple, time-tested and easy-to-follow.

The ChipShot™ Direct System provides quality reagents and enzymes for generating fluorescently labeled cDNA via direct incorporation of fluorescently modified nucleotides. Labeled cDNA then can be hybridized to a printed microarray.

The ChipShot™ Indirect System provides quality reagents...

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The ChipShot™ Labeling and Clean-Up Systems enable researchers to generate consistent, reproducible and sensitive microarray data. The ChipShot™ Systems make it easy for individual researchers to perform RNA and microarray manipulation, regardless of their experience. The protocols are simple, time-tested and easy-to-follow.

The ChipShot™ Direct System provides quality reagents and enzymes for generating fluorescently labeled cDNA via direct incorporation of fluorescently modified nucleotides. Labeled cDNA then can be hybridized to a printed microarray.

The ChipShot™ Indirect System provides quality reagents and enzymes for generating fluorescently labeled cDNA using a two-step labeling process that includes synthesis of aminoallyl-modified cDNA, followed by conjugation to the CyDye™ NHS ester. Labeled cDNA then can be hybridized to a printed microarray.

Note: Customers must supply CyDye™ nucleotides or NHS esters.

Features - Benefits

ChipShot™ Systems

• Conserve Sample: Optimized protocols for 5μg of total RNA or 1.5μg of poly(A)+ RNA per labeling reaction.
• Rely On Quality Products: Quality-controlled reagents are always available from Promega.
• Simplify Your Process: Easy-to-follow protocols.
• Increase Productivity: No hybridization reagent preparation and testing.
• Count On Technical Support: Outstanding technical support from Promega for the researcher.

ChipShot™ Direct System

• Increase Productivity: RNA to purified, labeled cDNA in approximately 3 hours.
• Get Reliable Results: Separate protocols for CyDye™3 and CyDye™5 labeling, so dye swap experiments are not necessary.

ChipShot™ Indirect System

• Achieve Higher Yields: Efficient incorporation of aminoallyl dNTP by reverse transcriptase yields more cDNA for labeling.
• Get Unbiased Dye Incorporation: CyDye™3- and CyDye™5-label cDNA at a comparable rate.

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