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Promega Corporation

Protein Interaction Analysis Tools

See All pGL4 Vectors
The CheckMate™/Flexi® Vector Mammalian-Two Hybrid System applies the principles of the yeast two-hybrid system to mammalian systems. Putative interacting proteins are inserted into the BIND (pFN11A) and ACT (pFN10A) vectors and transfected with the reporter construct, pGL4.31. If the proteins interact, they bring the GAL4 DNA Binding domain (BIND) in close proximity to the VP16 activation domain (ACT) initiating transcription on the pGL4.31 reporter construct, leading to generation of the firefly luciferase. Successful interactions can be assessed with the Dual-Luciferase® Reporter Ass...

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CheckMate™/Flexi® Vector Mammalian Two-Hybrid System

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CheckMate™/Flexi® Vector Mammalian Two-Hybrid System

 Components

  • pFN10A (ACT) Flexi® Vector

    C933B1 x 5μg
  • pFN11A (BIND) Flexi® Vector

    C934B1 x 5μg
  • pGL4.31[luc2P/GAL4UAS/Hygro] Vector

    C935A1 x 20μg
  • pBIND Vector

    E245A1 x 20μg
  • pACT Vector

    E246A1 x 20μg
  • pBIND-Id Control Vector

    E247A1 x 20μg
  • pACT-MyoD Control Vector

    E248A1 x 20μg
1 each
- C9360 $ 748.00 Add to cart

pGL4.31[luc2P/GAL4UAS/Hygro] Vector

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pGL4.31[luc2P/GAL4UAS/Hygro] Vector

 Components

  • pGL4.31[luc2P/GAL4UAS/Hygro] Vector

    C935A1 x 20μg
20μg
- C9351 $ 372.00 Add to cart

pFN10A (ACT) Flexi® Vector

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pFN10A (ACT) Flexi® Vector

 Components

  • pFN10A (ACT) Flexi® Vector

    C933A1 x 20μg
20μg
- C9331 $ 298.00 Add to cart

pFN11A (BIND) Flexi® Vector

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pFN11A (BIND) Flexi® Vector

 Components

  • pFN11A (BIND) Flexi® Vector

    C934A1 x 20μg
20μg
- C9341 $ 298.00 Add to cart

CheckMate™ Positive Control Vectors

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CheckMate™ Positive Control Vectors

 Components

  • pBIND-Id Control Vector

    E247A1 x 20μg
  • pACT-MyoD Control Vector

    E248A1 x 20μg
1 set
- C9370 $ 204.00 Add to cart

CheckMate™ Negative Control Vectors

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CheckMate™ Negative Control Vectors

 Components

  • pBIND Vector

    E245A1 x 20μg
  • pACT Vector

    E246A1 x 20μg
1 set
- C9380 $ 135.00 Add to cart


GenBank®/EMBL Accession Number

pGL4.31[luc2P/GAL4UAS/Hygro] Vector Accession Number  DQ487213; pGL4.31[luc2P/GAL4UAS/Hygro] Vector sequence text file.
pFN10A (ACT) Flexi® Vector Accession Number DQ487211; pFN10A (ACT) Flexi® Vector sequence text file.
pFN11A (BIND) Flexi® Vector Accession Number DQ487212; pFN11A (BIND) Flexi® Vector sequence text file.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

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