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High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample processing. Throughput rates of several thousand samples per hour may be achieved with high reproducibility under standard laboratory conditions.
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For more information, see the Protocols & Applications Guide.
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Steady-Glo® Luciferase Assay System
Steady-Glo® Luciferase Assay Substrate
Steady-Glo® Luciferase Assay Buffer
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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Store Steady-Glo® Luciferase Assay Substrate at –20°C. Store Steady-Glo® Luciferase Assay Buffer below 25°C.
For product intended use please see Patents & Disclaimers tab.
E2510, E2520, E2550 For Research Use Only. Not for Use in Diagnostic Procedures.
E2510, E2520, E2550 Certain applications of this product may require licenses from others.
E2510, E2520, E2550 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.
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