ONE-Glo™ Luciferase Assay System

The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideally suited for high- and ultrahigh-throughput applications, the ONE-Glo™ Assay contains a new luciferase substrate, resulting in a reagent that is more stable, more tolerant to sample components, and has less odor than standard luciferase assay reagents. These features ensure that the ONE-Glo™ Assay provides robust performance and also eliminates many of the handling inconveniences experienced using other reporter assays in a high-throughpu...

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The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideally suited for high- and ultrahigh-throughput applications, the ONE-Glo™ Assay contains a new luciferase substrate, resulting in a reagent that is more stable, more tolerant to sample components, and has less odor than standard luciferase assay reagents. These features ensure that the ONE-Glo™ Assay provides robust performance and also eliminates many of the handling inconveniences experienced using other reporter assays in a high-throughput setting.

Features - Benefits

• Simplify Your Assay Optimization: The robust performance, reduced odor, improved storage, and larger available sizes make assay optimization even easier and more efficient.
• Store at Room Temperature or at 4°C: The extended stability of the ONE-Glo™ Reagent at room temperature and 4°C makes it more convenient for everyday use.
• Improve Assay Precision: Because the ONE-Glo™ Reagent is less sensitive to mixing and dispensing conditions, reproducibility is enhanced. The assay is ideal for use in high-density (384- and 1536-well) microplates.
• Get a Brighter, Longer-Lasting Signal: Optimized for batch and continuous-process handling, the extended bright light output of the ONE-Glo™ Assay allows high sensitivity, especially when extended incubation is required prior to reading results.
• Reduce Unwanted Effects from Sample Components: The novel chemistry used in the ONE-Glo™ Assay is less sensitive to culture media, phenol red, and luciferase inhibitors than other luciferase assays.

Notes

Please contact Promega for information on bulk purchases and custom configurations.

Patents - Disclaimers

Patent Pending.

The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

Certain applications of this product may require licenses from others.

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