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The CAT Enzyme Assay System offers two alternative methods for monitoring CAT enzyme activity in transfected cells: liquid scintillation counting (LSC) and thin layer chromatography (TLC). Either the LSC or TLC assays can be performed using the same cell extract. The TLC-based assay is less sensitive and more time-consuming to perform than the LSC assay but is useful as a visual confirmation of assay results. The resolved TLC reaction products are detected by autoradiography or phosphorimaging analysis.
Chloramphenicol Acetyltransferase (CAT), encoded by a bacterial drug-resistance gene, catalyzes the transfe...
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Chloramphenicol Acetyltransferase (CAT), encoded by a bacterial drug-resistance gene, catalyzes the transfer of an acetyl group from acetyl-CoA to the 3´-hydroxy position of chloramphenicol. The enzyme is suitable as a standard in CAT assays of crude cell extracts. One unit is defined as the amount of enzyme required to transfer 1nmol of butyrate or acetate to chloramphenicol in one minute at 37°C.
n-Butyryl CoA is suitable for use in the chloramphenicol acetyltransferase (CAT) reaction. Transfer of the n-butyryl moiety to chloramphenicol by the CAT enzyme allows enzyme activity to be monitored using liquid scintillation counting or thin layer chromatography formats.
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CAT Enzyme Assay System
Reporter Lysis 5X Buffer
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.
Reporter Lysis 5X Buffer may be stored at room temperature. Store other system components at –20°C.
For product intended use please see Patents & Disclaimers tab.
E1000, E1051, E1061, E3971 For Research Use Only. Not for Use in Diagnostic Procedures.
E1000 Certain applications of this product may require licenses from others.
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