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Promega Corporation

Gel Shift Assay Systems

The gel shift or electrophoretic mobility shift assay provides a simple and rapid method for detecting DNA-binding proteins. This method is widely used to study sequence-specific DNA-binding proteins such as transcription factors. The assay is based on the observation that complexes of protein and DNA migrate through a nondenaturing polyacrylamide gel more slowly than free DNA fragments or double-stranded oligonucleotides. The gel shift assay is performed by incubating a purified protein or a complex mixture of proteins (such as nuclear or cell extract preparations) with a 32P end-labeled DNA fragment containing ...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

Gel Shift Assay Core System

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Gel Shift Assay Core System

 Components

  • Kinase 10X Buffer

    C131B1 x 100μl
  • AP2 Consensus Oligonucleotide

    E321B1 x 35pmol
  • SP1 Consensus Oligonucleotide

    E323B1 x 35pmol
  • HeLa Nuclear Extract

    E352A1 x 40μl
  • Gel Shift Binding 5X Buffer

    E358A1 x 200μl
  • T4 Polynucleotide Kinase

    M410A1 x 100u
100 reactions
- E3050 $ 342.00 Add to cart

Gel Shift Assay System

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Gel Shift Assay System

 Components

  • Kinase 10X Buffer

    C131B1 x 100μl
  • AP1 Consensus Oligonucleotide

    E320B1 x 35pmol
  • AP2 Consensus Oligonucleotide

    E321B1 x 35pmol
  • TFIID Consensus Oligonucleotide

    E322B1 x 35pmol
  • SP1 Consensus Oligonucleotide

    E323B1 x 35pmol
  • OCT1 Consensus Oligonucleotide

    E324B1 x 35pmol
  • CREB Consensus Oligonucleotide

    E328B1 x 35pmol
  • NF-κB Consensus Oligonucleotide

    E329B1 x 35pmol
  • HeLa Nuclear Extract

    E352A1 x 40μl
  • Gel Shift Binding 5X Buffer

    E358A1 x 200μl
  • T4 Polynucleotide Kinase

    M410A1 x 100u
100 reactions
- E3300 $ 426.00 Add to cart


Available Separately

Product Size Conc. Catalog # *List Price Order QTY Add to Cart

HeLaScribe® Nuclear Extract, Gel Shift Assay Grade

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HeLaScribe® Nuclear Extract, Gel Shift Assay Grade

  Components

  • HeLa Nuclear Extract

    E352A3 x 40μl
3 × 40μl
- E3521 $ 177.00 Add to cart

Gel Shift Binding 5X Buffer

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Gel Shift Binding 5X Buffer

  Components

  • Gel Shift Binding 5X Buffer

    E358A5 x 200μl
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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5 × 200μl
- E3581 $ 369.00 Add to cart

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Storage Conditions

Store HeLa Nuclear Extract at –70°C. Store other components at –20°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

E3050, E3300, E3521, E3581 For Research Use Only. Not for Use in Diagnostic Procedures.

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