HisLink™ Spin Protein Purification System

The HisLink™ Spin Protein Purification System provides a simple, quick and robust method of purifying polyhistidine- or HQ-tagged expressed proteins from E. coli using either a centrifuge- or vacuum-based method.

Proteins can be purified directly from culture medium containing bacterial cells expressing polyhistidine- or HQ-tagged protein. The bacterial cells are lysed using the FastBreak™ Cell Lysis Reagent, followed immediately by the addition of the HisLink™ Protein Purification Resin to the culture. The addition of these reagents results in simultaneous bacterial lysis and binding of the polyhistidine- o...

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The HisLink™ Spin Protein Purification System provides a simple, quick and robust method of purifying polyhistidine- or HQ-tagged expressed proteins from E. coli using either a centrifuge- or vacuum-based method.

Proteins can be purified directly from culture medium containing bacterial cells expressing polyhistidine- or HQ-tagged protein. The bacterial cells are lysed using the FastBreak™ Cell Lysis Reagent, followed immediately by the addition of the HisLink™ Protein Purification Resin to the culture. The addition of these reagents results in simultaneous bacterial lysis and binding of the polyhistidine- or HQ-tagged proteins. Samples are then transferred to a Spin Column, where unbound protein is washed away and the target protein of interest is recovered by elution.

Features - Benefits

• Versatile: Choose between vacuum or centrifugation formats.
• Convenient: Complete system containing all necessary components.
• Productive: High binding capacity per column.

Applications

• Protein purification of multiple polyhistidine- or HQ-tagged expressed proteins from E. coli using a vacuum- or centrifugation-based method.

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