Promega Corporation

HisLink™ 96 Protein Purification System

HisLink™ 96 Protein Purification System provides a simple, quick and robust method of purifying multiple polyhistidine- or HQ-tagged expressed proteins from E. coli using a vacuum-based method. The system is designed to purify expressed polyhistidine- or HQ-tagged proteins directly from deep-well, 96-well plates. The HisLink™ 96 System is amenable to manual or automated methods, such as the Beckman Coulter Biomek® 2000 or FX for high-throughput applications.

Using the provided FastBreak™ Cell Lysis Reagent, bacterial cells containing polyhistidine- or HQ-tagged protein are lysed directly in culture. The HisLink...

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HisLink™ 96 Protein Purification System

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HisLink™ 96 Protein Purification System

 Components

  • HisLink™ 96 Filtration/Collection Plates

    V484A1 x 1 pack
  • Binding/Wash Buffer

    V851C1 x 110ml
  • Elution Buffer

    V852C1 x 25ml
  • FastBreak™ Cell Lysis Reagent, 10X

    V857A1 x 15ml
  • HisLink™ Protein Purification Resin

    V882B1 x 25ml
  • DNase I (lyophilized)

    Z358B1 x 1 vial
1 × 96
- V3680 $ 300.00 Add to cart

HisLink™ 96 Protein Purification System

Close Window

HisLink™ 96 Protein Purification System

 Components

  • HisLink™ 96 Filtration/Collection Plates

    V484A5 x 1 pack
  • Binding/Wash Buffer

    V851D2 x 280ml
  • Elution Buffer

    V852D1 x 125ml
  • FastBreak™ Cell Lysis Reagent, 10X

    V857B1 x 60ml
  • HisLink™ Protein Purifiication Resin

    V882A2 x 50ml
  • DNase I (lyophilized)

    Z358A1 x 1 vial
5 × 96
- V3681 $ 1,262.00 Add to cart


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Storage Conditions

Store the system at 4°C. Plates may be stored at 4°C or room temperature.

For product intended use please see Patents & Disclaimers tab.

Figures

Overview of polyhistidine-tagged protein purification using the HisLink 96 Protein Purification System.Figure 1. Overview of polyhistidine-tagged protein purification using the HisLink™ 96 Protein Purification System.

Use Restrictions

V3680, V3681 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

U.S. Pat. Nos. 7,112,552 and 7,354,750.

This product is licensed for use under U.S. Pat. No. 6,174,704.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

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