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Promega Corporation

HaloTag® Mammalian Pull-Down Systems

The HaloTag® Mammalian Pull-Down Systems (Cat.# G6500, G6504 and G6509) are designed to capture and purify intracellular binary and higher order protein complexes, including transient or weakly interacting partners.

HaloTag® Mammalian Pull-Down System (Cat.# G6504) includes buffers and resin necessary to perform a HaloTag® pull-down.

HaloTag® Mammalian Pull-Down and Labeling System (Cat.# G6500) includes everything in G6504 plus the HaloTag® TMRDirect™ Ligand, which allows correlative cellular localization and real-time imaging studies.

HaloTag® Complete Pull-Down System (Cat.# G6509) ...

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  • Prices valid for US customers only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart
HaloTag® Complete Pull-Down System
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HaloTag® Complete Pull-Down System  Components

  • Wizard® SV Gel and PCR Clean-Up System

    A92801 x 10 preps
  • HaloTag® Pull-Down Cloning System

    G60511 x 40 reactions
  • FuGENE® HD Transfection Reagent

    E23111 x 1ml
  • HaloTag® Mammalian Pull-Down System

    G65011 x 1 system
  • Protease Inhibitor Cocktail, 50X

    G65211 x 1ml
  • HaloTag® TMRDirect™ Ligand

    G29911 x 30μl
1 each
- G6509 $ 995.00 Add to cart

HaloTag® Mammalian Pull-Down and Labeling System

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HaloTag® Mammalian Pull-Down and Labeling System

 Components

  • HaloTag® TMRDirect™ Ligand

    G299A1 x 30μl
  • 10X TBS Buffer

    G650A1 x 25ml
  • SDS Elution Buffer

    G651A1 x 1.3ml
  • Mammalian Lysis Buffer

    G938B1 x 10ml
  • HaloLink™ Resin

    G19121 x 1.25ml
  • Protease Inhibitor Cocktail, 50X

    G652A1 x 1ml
24 reactions
- G6500 $ 384.00 Add to cart

HaloTag® Mammalian Pull-Down System

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HaloTag® Mammalian Pull-Down System

 Components

  • 10X TBS Buffer

    G650A1 x 25ml
  • SDS Elution Buffer

    G651A1 x 1.3ml
  • Mammalian Lysis Buffer

    G938B1 x 10ml
  • HaloLink™ Resin

    G19121 x 1.25ml
  • Protease Inhibitor Cocktail, 50X

    G652A1 x 1ml
24 reactions
- G6504 $ 274.00 Add to cart

HaloTag® Control Vector

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HaloTag® Control Vector

 Components

  • HaloTag® Control Vector

    G659A1 x 20μg
20μg
- G6591 $ 184.00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

Protease Inhibitor Cocktail, 50X

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Protease Inhibitor Cocktail, 50X

 Components

  • Protease Inhibitor Cocktail, 50X

    G652A1 x 1ml
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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1ml
- G6521 $ 65.00 Add to cart

Mammalian Lysis Buffer

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Mammalian Lysis Buffer

 Components

  • Mammalian Lysis Buffer

    G938A1 x 40ml
40ml
- G9381 $ 46.00 Add to cart

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GenBank®/EMBL Accession Number

HaloTag® Control Vector Accession Number HM157289; HaloTag® Control Vector sequence text file.

Storage Conditions

Store the 10X TBS Buffer and SDS Elution Buffer at room temperature. Store the HaloLink™ Resin and Mammalian Lysis Buffer at 4°C. Store the HaloTag® TMRDirect™ Ligand and powdered Protease Inhibitor Cocktail at –30 to –10°C. Reconstituted Protease Inhibitor Cocktail can be stored at 2–10°C  for 12 months.

For product intended use please see Patents & Disclaimers tab.

Representation of the HaloTag mammalian pull-down assay using HaloTag fusion protein as bait.Figure 1. Representation of the HaloTag® mammalian pull-down assay using HaloTag® fusion protein as bait.

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Use Restrictions

G6509, G6500, G6504, G6591, G6521, G9381 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G6509 FuGENE® HD is sold with a Limited Use Label License (LULL).
You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

G6509, G6500, G6504 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may only use this product in their own research and may modify or link Promega HaloTag® ligands to Promega or customer-supplied moieties. No other use or transfer of this product is authorized without the express, written consent of Promega including, without limitation, commercial use. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

G6509, G6591 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

G6509 European Pat. No. 1685247 and other patents pending.

G6509, G6591 U.S. Pat. Nos. 7,425,436 and 7,935,803 and other patents pending.

G6509 U.S. Pat. Nos. 7,429,472, 7,888,086, 8,202,700 and other patents pending.

G6509 Patent Pending.

G6509, G6591 For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

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