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The HaloTag® Fluorescent Ligands can carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under general physiological conditions, is highly specific and essentially irreversible. The HaloTag® Fluorescent Ligands allow researchers to apply the chloroalkane group that HaloTag® protein reacts with to any compound or surface with a compatible chemical group, creating endless possible applications.
HaloTag® Fluorescent Ligands for Cellular Imaging
Cell-permeant fluorescent ligands (rapid labeling protocol):
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Cell-impermeant fluorescent ligands for cell-surface labeling (rapid labeling protocol):
Cell-permeant fluorescent ligands ("no wash" protocol):
The Alexa Fluor® 488 Ligand is impermeable to cell membranes and, therefore, used to label cell surface proteins. The TMR Ligand, Oregon Green® Ligand, diAcFAM Ligand and Coumarin Ligand readily cross the cell membrane and, therefore, can be used to label intracellular proteins.
HaloTag® Ligands for Protein Detection
The HaloTag® Biotin Ligand consists of a 12-atom linker arm to biotin and is used as an affinity tag to capture the HaloTag® protein-based fusion construct using the strong biotin-streptavidin interaction.
The HaloTag® PEG-Biotin Ligand contains a spacer not found in the HaloTag® Biotin Ligand. This provides a significantly longer and more flexible linker between streptavidin and the HaloTag® protein, which may be advantageous in preserving the activity of a HaloTag® fusion partner protein upon immobilization or derivatization.
For more information, see the Protocols & Applications Guide.
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HaloTag® TMR Ligand
HaloTag® Oregon Green® Ligand
HaloTag® diAcFAM Ligand
HaloTag® Coumarin Ligand
HaloTag® Alexa Fluor® 488 Ligand
HaloTag® TMRDirect™ Ligand
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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HaloTag® R110Direct™ Ligand
HaloTag® Biotin Ligand
HaloTag® PEG-Biotin Ligand
For product intended use please see Patents & Disclaimers tab.
All HaloTag® Ligands have the same chloroalkane reactive linker. The ligands differ in the functional reporter and in the distance of the reporter from the linker. HaloTag® Alexa Fluor® 488 Ligand structure reprinted with permission of Molecular Probes. HaloTag® Alexa Fluor® 660 Ligand structure not available.
G8251, G8252, G2801, G2802, G8272, G8273, G8581, G8582, G1001, G1002, G8471, G8472, G2991, G3221, G8281, G8282, G8591, G8592 For Research Use Only. Not for Use in Diagnostic Procedures.
G8251, G8252, G2801, G2802, G8272, G8273, G8581, G8582, G1001, G1002, G8471, G8472, G2991, G3221, G8281, G8282, G8591, G8592 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may only use this product in their own research and may modify or link Promega HaloTag® ligands to Promega or customer-supplied moieties. No other use or transfer of this product is authorized without the express, written consent of Promega including, without limitation, commercial use. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
G8251, G8252, G2801, G2802, G8272, G8273, G8581, G8582, G1001, G1002, G8471, G8472, G2991, G3221 U.S. Pat. No. 7,867,726 and other patents and patents pending.
G8281, G8282, G8591, G8592 U.S. Pat. No. RE42931 and other patents and patents pending.
G2801, G2802, G1001, G1002, G8471, G8472 The fluorescent dye (the "Dye") of this product (the "Product") is provided under an agreement between Molecular Probes, Inc., and Promega Corporation, and the manufacture, use, sale or import of this Product is subject to: (i) one or more of US Patents and pending US applications and corresponding international equivalents owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corporation); and (ii) one or more of US Patents and pending US applications and corresponding international equivalents owned by Promega Corporation. The purchase of this Product conveys to the buyer the non-transferable right to use the purchased amount of the Product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The use of this Product for providing medical or diagnostic results or services, including without limitation testing, analysis, or screening, or for providing clinical information or clinical analysis in return for compensation on a per-test basis, is expressly excluded. The buyer cannot sell or otherwise transfer (a) this Product, (b) its components or (c) materials made using this Product or its components to a third party or otherwise use this Product or its components or materials made using this Product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include but is not limited to: (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data; (3) use of the Product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. For information on purchasing a license to the Dye for purposes other than as set forth above, contact Molecular Probes, Inc., Business Development, 29851 Willow Creek Road, Eugene, OR 97402-9132. Tel: (541) 465-8300. Fax: (541) 335-0504. For information on purchasing a license to this Product for purposes other than as set forth above, contact Promega Corporation.
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