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The FluoroTect™ GreenLys in vitro Translation Labeling System allows for the fluorescent labeling and detection of proteins synthesized in vitro. The system is based on a lysine-charged tRNA that is labeled at the ε position of the lysine with the fluorophore BODIPY®-FL. Fluorescent lysine residues will be incorporated into synthesized proteins during in vitro translation reactions, eliminating the need for radioactivity.
Detection of the labeled proteins is accomplished in 2–5 minutes directly "in-gel" by use of a laser-based fluorescent gel scanner. This eliminates any requirements for protein gel manipulatio...
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Detection of the labeled proteins is accomplished in 2–5 minutes directly "in-gel" by use of a laser-based fluorescent gel scanner. This eliminates any requirements for protein gel manipulation such as fixing/drying or any safety, regulatory and waste disposal issues associated with the use of radioactively labeled amino acids use. The convenience of "in-gel" detection also avoids the time-consuming electroblotting and detection steps of conventional non-isotopic systems.
For more information, see the Protocols & Applications Guide.
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FluoroTect™ GreenLys in vitro Translation Labeling System
FluoroTect™ GreenLys tRNA
Store at –70°C.
For product intended use please see Patents & Disclaimers tab.
L5001 For Research Use Only. Not for Use in Diagnostic Procedures.
L5001 FluoroTect™ GreenLys incorporates Fluorotag® technology, which is licensed under U.S. Pat. Nos. 6,306,628 and 7,252,932. Commercial use of this product requires a license from AmberGen, Inc. Fluorotag is a registered trademark of AmberGen, Inc.
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