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Promega Corporation

S30 T7 High-Yield Protein Expression System

The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500μg/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site. The protein expression system provides an extract that contains T7 RNA polymerase for transcription and is deficient in OmpT endoproteinase and lon protease activity. All other necessary components in the system are optimized for protein expression. This results in greater stability and enhanced expression of target proteins.

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S30 T7 High-Yield Protein Expression System

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S30 T7 High-Yield Protein Expression System

 Components

  • S30 Premix Plus

    L111A3 x 200μl
  • S30 T7 Control DNA

    L112A1 x 20μl
  • T7/S30 Extract for Circular DNA

    L114A3 x 150μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
24 reactions
- L1110 $ 426.00 Add to cart

S30 T7 High-Yield Protein Expression System

Close Window

S30 T7 High-Yield Protein Expression System

 Components

  • S30 Premix Plus

    L111A1 x 200μl
  • S30 T7 Control DNA

    L112A1 x 20μl
  • T7/S30 Extract for Circular DNA

    L114A1 x 150μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
8 reactions
- L1115 $ 169.00 Add to cart


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Storage Conditions

Store at –70°C.

For product intended use please see Patents & Disclaimers tab.

9695MF_500Figure 1. Representation of the Protein Expression System.

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Protein synthesis using the T7 S30 High-Yield Protein Expression System.Figure 2. Protein synthesis using the T7 S30 High-Yield Protein Expression System.

All protein coding sequences were cloned into pFN6A and expressed using 1µg of plasmid DNA. Lane 1, no DNA; lane 2, pFN6AhRl; lane 3, pFN6A-HT2; lane 4, pFN6A-β-gal.

Use Restrictions

L1110, L1115 For Research Use Only. Not for Use in Diagnostic Procedures.

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