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Promega Corporation

E. coli S30 Extract System for Circular DNA

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The E. coli S30 Extract for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or λ vectors, providing a powerful tool for identifying and characterizing polypeptides. The investigator needs only to supply the cloned DNA containing the appropriate prokaryotic promoter and ribosome binding sites. The S30 Extract for Circular DNA Templates is prepared by modifications of the method described by Zubay from an E. coli strain B deficient in OmpT endoproteinase and lon protease activity. This results in a greater stability of expressed proteins that would otherwise be degraded by pr...

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E. coli S30 Extract System for Circular DNA

30 reactions
- L1020 $ 403.00 Add to cart


Storage Conditions

Store at –70°C.

For product intended use please see Patents & Disclaimers tab.

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