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The TnT® SP6 High-Yield Wheat Germ Protein Expression System, based on an optimized wheat germ extract, is a single-tube, coupled transcription/translation system designed to express proteins in only two hours. Protein synthesized, in the range of 10–100μg/ml, can be used in multiple proteomic-based applications, as well as in high-throughput analysis.
All components necessary for transcription/translation are provided in the extract, with the exception of the plasmid DNA or PCR template. Optional protein-labeling reagents must also be supplied by the user.
For custom wheat germ extract (depleted amino ...
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For custom wheat germ extract (depleted amino acids), order Cat.# X808X (see Products, Available Separately).
For more information, see the Protocols & Applications Guide.
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TnT® SP6 High-Yield Wheat Germ Protein Expression System
TnT® SP6 High-Yield Wheat Germ Master Mix
TnT® SP6 High-Yield Master Mix Minus Amino Acids
Store at –70°C.
For product intended use please see Patents & Disclaimers tab.
Proteins were expressed by dialysis reaction using 8µg of plasmid DNA. Lane 1, firefly luciferase (62kDa); lane 2, GFP (28kDa); lane 3, Renilla luciferase (36kDa). For additional details regarding the dialysis reaction, please see the technical article.
L3260, L3261, X808X For Research Use Only. Not for Use in Diagnostic Procedures.
L3260, L3261 Any use of the product for diagnostics requiring clearance or approval by the U.S. Food and Drug Administration or a foreign equivalent may require a license under Mayo Clinic U.S. Pat. No. 6,027,913.
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