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Promega Corporation

TnT® SP6 High-Yield Wheat Germ Protein Expression System

The TnT® SP6 High-Yield Wheat Germ Protein Expression System, based on an optimized wheat germ extract, is a single-tube, coupled transcription/translation system designed to express proteins in only two hours. Protein synthesized, in the range of 10–100μg/ml, can be used in multiple proteomic-based applications, as well as in high-throughput analysis.

All components necessary for transcription/translation are provided in the extract, with the exception of the plasmid DNA or PCR template. Optional protein-labeling reagents must also be supplied by the user.

For custom wheat germ extract (depleted amino ...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

TnT® SP6 High-Yield Wheat Germ Protein Expression System

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TnT® SP6 High-Yield Wheat Germ Protein Expression System

 Components

  • TnT® SP6 High-Yield Wheat Germ Master Mix

    L326A4 x 300μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
40 reactions
- L3260 $ 593.00 Add to cart

TnT® SP6 High-Yield Wheat Germ Protein Expression System

Close Window

TnT® SP6 High-Yield Wheat Germ Protein Expression System

 Components

  • TnT® SP6 High-Yield Wheat Germ Master Mix

    L326A1 x 300μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
10 reactions
- L3261 $ 184.00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

TnT® SP6 High-Yield Master Mix Minus Amino Acids

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TnT® SP6 High-Yield Master Mix Minus Amino Acids

 Components


- X808X $ 600.00 Add to cart

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Storage Conditions

Store at –70°C.

For product intended use please see Patents & Disclaimers tab.

9695ME_500Figure 1. Representation of the Protein Expression System.

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Protein synthesis in TNT High-Yield Wheat Germ Protein Expression System.Figure 2. Protein synthesis in TnT® High-Yield Wheat Germ Protein Expression System.

Proteins were expressed by dialysis reaction using 8µg of plasmid DNA. Lane 1, firefly luciferase (62kDa); lane 2, GFP (28kDa); lane 3, Renilla luciferase (36kDa). For additional details regarding the dialysis reaction, please see the technical article.

Use Restrictions

L3260, L3261, X808X For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

L3260, L3261 Any use of the product for diagnostics requiring clearance or approval by the U.S. Food and Drug Administration or a foreign equivalent may require a license under Mayo Clinic U.S. Pat. No. 6,027,913.

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