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The TnT® Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression. These cell-free expression systems combine the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin® Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TnT® Quick Master Mix.
The TnT® Quick Coupled Transcription/Translation System is available in two configurations for the expression of genes cloned downstream from either the T7 or SP6 RNA polymerase promoters. To use these cell-free expression systems, 0.2–2.0μg of circular p...
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The TnT® Quick Coupled Transcription/Translation System is available in two configurations for the expression of genes cloned downstream from either the T7 or SP6 RNA polymerase promoters. To use these cell-free expression systems, 0.2–2.0μg of circular plasmid DNA containing a T7 or SP6 promoter, or a PCR-generated fragment containing a T7 promoter, is added to an aliquot of the TnT® Quick Master Mix and incubated in a 50μl reaction volume for 60–90 minutes at 30°C. The expression reaction produces significant quantities of protein for a variety of applications including GST pull-downs and gel shift assays.
For more information about cell-free protein expression, see the Protein Expression chapter of the Protocols & Applications Guide.
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TnT® T7 Quick Coupled Transcription/Translation System
T7 TnT® PCR Enhancer
Luciferase T7 Control DNA
Luciferase Assay Reagent
TnT® T7 Quick Master Mix
TnT® T7 Quick Coupled Transcription/Translation System, Trial Size
TnT® SP6 Quick Coupled Transcription/Translation System
TnT® SP6 Quick Master Mix
Luciferase SP6 Control DNA
TnT® SP6 Quick Coupled Transcription/Translation System, Trial Size
Store at –70°C. Do not freeze-thaw the lysate more than two times.
For product intended use please see Patents & Disclaimers tab.
L1170, L1171, L2080, L2081, L4581, L4591 For Research Use Only. Not for Use in Diagnostic Procedures.
L1170, L1171 Any use of the product for diagnostics requiring clearance or approval by the U.S. Food and Drug Administration or a foreign equivalent may require a license under Mayo Clinic U.S. Pat. No. 6,027,913.
L1170, L1171, L2080, L2081 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
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