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The T7 Sample System is designed to facilitate the optimization of individual gene expression by offering four unique in vitro translation systems to evaluate. The system consists of samples of: TnT® T7 Quick for PCR DNA, TnT® T7 Quick Coupled Transcription/Translation System, TnT® Coupled Wheat Germ Extract System and E. coli T7 S30 Extract System for Circular DNA.
All of the coupled systems utilize RNA generated by a T7 phage promoter. Criteria such as post-translational modifications, ionic optimization and detection methods (i.e., non-isotopic) should be considered when choosing an in vitro system. In some ...
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All of the coupled systems utilize RNA generated by a T7 phage promoter. Criteria such as post-translational modifications, ionic optimization and detection methods (i.e., non-isotopic) should be considered when choosing an in vitro system. In some cases only direct experimental results will confirm which system is best for specific genes.
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T7 Sample System
T7/S30 Extract for Circular DNA
TnT® Wheat Germ Extract
Amino Acid Mixture, Complete
TnT® Reaction Buffer
Luciferase T7 Control DNA
S30 Premix without Amino Acids
TnT® T7 Wheat Germ Polymerase
TnT® T7 Quick Master Mix
TnT® T7 PCR Quick Master Mix
Amino Acid Mixture Minus Methionine
Store at –70°C.
For product intended use please see Patents & Disclaimers tab.
L5900 For Research Use Only. Not for Use in Diagnostic Procedures.
L5900 Any use of the product for diagnostics requiring clearance or approval by the U.S. Food and Drug Administration or a foreign equivalent may require a license under Mayo Clinic U.S. Pat. No. 6,027,913.
L5900 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
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