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The Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System contains both Rabbit Reticulocyte Lysate and Wheat Germ Extract for comparing in vitro translation systems. Reticulocyte Lysate is prepared from New Zealand white rabbits. The Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cellular debris. Both systems contain the cellular components necessary for protein synthesis. The systems have been treated with micrococcal nuclease, which destroys endogenous mRNA and results in minimal background translation.
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For more information, see the Protein Expression chapter of the Protocols & Applications Guide.
Each system contains 2 × 200μl of both Rabbit Reticulocyte Lysate and Wheat Germ Extract; this is sufficient for 12 reactions using the Rabbit Reticulocyte Lysate and 12 reactions using the Wheat Germ Extract.
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Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System
Reticulocyte Lysate, Nuclease Treated
Wheat Germ Extract
Amino Acid Mixture Minus Cysteine
Luciferase Control RNA
Amino Acid Mixture Minus Leucine
Amino Acid Mixture Minus Methionine
Store at –70°C or below. Do not freeze-thaw the lysate more than two times.
For product intended use please see Patents & Disclaimers tab.
L4330 For Research Use Only. Not for Use in Diagnostic Procedures.
L4330 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
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