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The Flexi® Rabbit Reticulocyte Lysate System allows translation reactions to be optimized for a wide range of parameters, including Mg2+ and K+ concentrations and the choice of adding DTT. To help optimize Mg2+ for a specific message, the endogenous Mg2+ concentration of each lysate batch is stated in the product information included with this product. The Flexi® System also offers the choice of three amino acid mixtures and includes a control RNA encoding the firefly luciferase gene.
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For more information, see the Protocols & Applications Guide.
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Flexi® Rabbit Reticulocyte Lysate System
Amino Acid Mixture Minus Cysteine
Flexi® Rabbit Reticulocyte Lysate
Luciferase Control RNA
Luciferase Assay Wells
Luciferase Assay Reagent
Amino Acid Mixture Minus Leucine
Amino Acid Mixture Minus Methionine
Store at –70°C, except Luciferase Assay Wells, which can be stored at room temperature. Do not freeze-thaw the lysate more than two times.
For product intended use please see Patents & Disclaimers tab.
L4540 For Research Use Only. Not for Use in Diagnostic Procedures.
U.S. Pat. Nos. 5,641,641 and 5,650,289.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
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