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Promega Corporation

E. coli T7 S30 Extract System for Circular DNA

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The E. coli T7 S30 Extract System for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or λ vectors containing a T7 promoter by providing an extract that contains T7 RNA polymerase for transcription and all components needed for translation. The investigator only supplies cloned DNA containing a T7 promoter and a ribosome binding site. This product is prepared by modifications of the method described by Zubay from an E. coli strain B deficient in OmpT endoproteinase and lon protease activity. This results in greater stability of expressed proteins that would otherwise be deg...

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E. coli T7 S30 Extract System for Circular DNA

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E. coli T7 S30 Extract System for Circular DNA

 Components

  • T7/S30 Extract for Circular DNA

    L114A3 x 150μl
  • Amino Acid Mixture Minus Cysteine

    L447A1 x 175μl
  • S30 Premix without Amino Acids

    L512A1 x 750μl
  • Amino Acid Mixture Minus Leucine

    L995B1 x 175μl
  • Amino Acid Mixture Minus Methionine

    L996B1 x 175μl
  • PinPoint™ Xa Control Vector

    V204A1 x 5μg
30 reactions
- L1130 $ 424.00 Add to cart


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Storage Conditions

Store extract at –70°C. Check individual components for storage temperatures.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

L1130 For Research Use Only. Not for Use in Diagnostic Procedures.

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