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Promega Corporation

TnT® Quick Coupled Transcription/Translation System

The TnT® Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression. These cell-free expression systems combine the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin® Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TnT® Quick Master Mix.

The TnT® Quick Coupled Transcription/Translation System is available in two configurations for the expression of genes cloned downstream from either the T7 or SP6 RNA polymerase promoters. To use these cell-free expression systems, 0.2–2.0μg of circular p...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

TnT® T7 Quick Coupled Transcription/Translation System

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TnT® T7 Quick Coupled Transcription/Translation System

 Components

  • Methionine

    L118A1 x 50μl
  • T7 TnT® PCR Enhancer

    L216A1 x 100μl
  • Luciferase T7 Control DNA

    L482A1 x 5μg
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • TnT® T7 Quick Master Mix

    L520A8 x 200μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
40 reactions
- L1170 $ 528.00 Add to cart

TnT® T7 Quick Coupled Transcription/Translation System, Trial Size

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TnT® T7 Quick Coupled Transcription/Translation System, Trial Size

 Components

  • Methionine

    L118A1 x 50μl
  • T7 TnT® PCR Enhancer

    L216A1 x 100μl
  • Luciferase T7 Control DNA

    L482A1 x 5μg
  • TnT® T7 Quick Master Mix

    L520A1 x 200μl
5 reactions
- L1171 $ 144.00 Add to cart

TnT® SP6 Quick Coupled Transcription/Translation System

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TnT® SP6 Quick Coupled Transcription/Translation System

 Components

  • Methionine

    L118A1 x 50μl
  • TnT® SP6 Quick Master Mix

    L209A8 x 200μl
  • Luciferase SP6 Control DNA

    L474A1 x 5μg
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
40 reactions
- L2080 $ 528.00 Add to cart

TnT® SP6 Quick Coupled Transcription/Translation System, Trial Size

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TnT® SP6 Quick Coupled Transcription/Translation System, Trial Size

 Components

  • Methionine

    L118A1 x 50μl
  • TnT® SP6 Quick Master Mix

    L209A1 x 200μl
  • Luciferase SP6 Control DNA

    L474A1 x 5μg
5 reactions
- L2081 $ 145.00 Add to cart

Magnesium Acetate

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Magnesium Acetate

 Components

  • Magnesium Acetate

    L458A1 x 100μl
100μl
25mM L4581 $ 45.00 Add to cart

Potassium Chloride

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Potassium Chloride

 Components

  • Potassium Chloride

    L459A1 x 200μl
200μl
2.5M L4591 $ 45.00 Add to cart


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Storage Conditions

Store at –70°C. Do not freeze-thaw the lysate more than two times.

For product intended use please see Patents & Disclaimers tab.

Schematic diagram illustrating how the TNT® Quick System functions.Figure 1. Schematic diagram illustrating how the TnT® Quick System functions.

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Use Restrictions

L1170, L1171, L2080, L2081, L4581, L4591 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

L1170, L1171 Any use of the product for diagnostics requiring clearance or approval by the U.S. Food and Drug Administration or a foreign equivalent may require a license under Mayo Clinic U.S. Pat. No. 6,027,913.

L1170, L1171, L2080, L2081 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

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