ProtoBlot® II AP Systems with Stabilized Substrate and Western Express® Fast Blotting...

The ProtoBlot® II AP Systems with Stabilized Substrate are designed for the rapid and sensitive detection of proteins or other macromolecular antigens immobilized on nitrocellulose or PVDF membranes. Proteins can be transferred from gels after electrophoresis (Western blots) or bound directly from solution ("dot" blots). The Western Express® Fast Blotting Protocol is included with the system and can reduce dramatically the time required to perform immunodetection. All ProtoBlot® II AP Systems contain BSA as a stabilizer and 0.05% sodium azide as a preservative.

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The ProtoBlot® II AP Systems with Stabilized Substrate are designed for the rapid and sensitive detection of proteins or other macromolecular antigens immobilized on nitrocellulose or PVDF membranes. Proteins can be transferred from gels after electrophoresis (Western blots) or bound directly from solution ("dot" blots). The Western Express® Fast Blotting Protocol is included with the system and can reduce dramatically the time required to perform immunodetection. All ProtoBlot® II AP Systems contain BSA as a stabilizer and 0.05% sodium azide as a preservative.

Features - Benefits

• Fast: Easy-to-use Western Express® Protocol allows the detection of dot blots in 30–45 minutes and the detection of Western blots in 1–2 hours.
• Convenient: The system contains Western Blue® Stabilized Substrate for AP, which is a ready-to-use solution of BCIP/NBT. No reagent preparation is required for the substrate.

For many applications, AP conjugates are superior to HRP conjugates because they:

• offer greater sensitivity (tenfold) of detection.
• are not inhibited by azide.
• use a substrate that is not prone to fading during long-term storage.
• have protocols provided for both PVDF and nitrocellulose membranes.

References

1. Towbin, H. et al. (1979) Proc. Natl. Acad. Sci. USA 76, 4350–4.
2. Burnette, W.N. (1981) Anal. Biochem. 112, 195–203.
3. Hawkes, R. et al. (1982) Anal. Biochem. 119, 142–7.
4. Larson, G. (1992) Promega Notes 35, 18–20.
5. Blake, M.S. et al. (1984) Anal. Biochem. 136, 175–9.

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