Trypsin Gold, Mass Spectrometry Grade

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in additional peptide fragments that could interfere with database analysis of the mass of fragments detected by mass spectrometry. Trypsin Gold, Mass Spectrometry Grade, has been manufactured to provide maximum spe...

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Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in additional peptide fragments that could interfere with database analysis of the mass of fragments detected by mass spectrometry. Trypsin Gold, Mass Spectrometry Grade, has been manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin. The treated trypsin is then purified by affinity chromatography and lyophilized to yield Trypsin Gold, Mass Spectrometry Grade. Trypsin is often used for in-gel digestion. The digestion products are purified and concentrated, then analyzed by mass spectrometry to determine their molecular weights. Database searches can then be performed, using the mass of the peptides to identify the protein(s) resolved on the gel. Each lot of quality-tested Trypsin Gold, Mass Spectrometry Grade, is qualified for use with in-gel digestion and mass spectrometric analysis.

Features - Benefits

• Pure: TPCK treatment followed by affinity purification.
• Application Qualified: Each lot is qualified by mass spectrometry.
• Convenient: Provided in one vial.
• Good Value: Stability ensured up to five freeze-thaw cycles, thus minimizing leftover reagents.
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

• Tryptic digestion of peptides from 2-D gels.

For more information, see the Protein Purification and Analysis chapter of the Protocols and Applications Guide.

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