The TnT® SP6 High-Yield Wheat Germ Protein Expression System, based on an optimized wheat germ extract, is a single-tube, coupled transcription/translation system designed to express proteins in only two hours. Protein synthesized, in the range of 10–100μg/ml, can be used in multiple proteomic-based applications, as well as in high-throughput analysis.
All components necessary for transcription/translation are provided in the extract, with the exception of the plasmid DNA or PCR template. Optional protein-labeling reagents must also be supplied by the user.
For custom wheat germ extract (depleted amino ...
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The TnT® SP6 High-Yield Wheat Germ Protein Expression System, based on an optimized wheat germ extract, is a single-tube, coupled transcription/translation system designed to express proteins in only two hours. Protein synthesized, in the range of 10–100μg/ml, can be used in multiple proteomic-based applications, as well as in high-throughput analysis.
All components necessary for transcription/translation are provided in the extract, with the exception of the plasmid DNA or PCR template. Optional protein-labeling reagents must also be supplied by the user.
For custom wheat germ extract (depleted amino acids), order Cat.# X808X (see Products, Available Separately).
Features - Benefits
- Save Time: You can generate protein in only two hours, as compared to days when using cell-based (E. coli) systems.
- Choose Your Format: Use plasmid or PCR-generated templates to generate protein.
- Achieve High Yields: Generate 10- to 20-fold more protein (10–100μg/ml) when compared to other cell-free systems.
- Generate Usable Protein: Generate soluble, full-length protein and avoid problems associated with E. coli systems.
Applications
- Protein:protein interaction.
- Protein:nucleic acid interactions.
- Protein:ligand interaction.
- Enzyme assays.
- Protein expression.
For more information, see the Protocols & Applications Guide.
References
- Zhao, L. et al. (2010) J. Struct. Funct. Genomics 11, 201–9.
- Zhao, K.Q., Hurst, R., Slater, M.R. and Bulleit, R.F. (2007) J. Struct. Funct. Genomics 8, 199–208.
Patents - Disclaimers
U.S. Pat. Nos. 5,324,637 and 5,492,817 and other patents.
Any use of the product for diagnostics requiring clearance or approval by the U.S. Food and Drug Administration or a foreign equivalent may require a license under Mayo Clinic U.S. Pat. No. 6,027,913.
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