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Promega Corporation

UGT-Glo™ Assay

A Bright New Way to Measure UGT Activity

  • Speed: The luminescent format eliminates the need for time-consuming analyses such as HPLC and LC/MS.
  • Simplified Method: The simple "add and read" protocol makes the assay amenable to high-throughput screening in multiwell plates.
  • Sensitive: Requires less enzyme and allows you to scale down reaction volumes which saves on reagent costs.
  • No Fluorescence Interference: The assay system eliminates fluorescence interference by using luminescence to monitor enzyme activity.
  • Low False-Positive Rate: Use of a proprietary stabilized firefly luciferase (Ultra-Glo™ Recombinant Luciferase) and a proprietary luciferase buffer formulation minimizes the incidence of false positives due to luciferase inhibition.

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Conversion of UGT Multienzyme Substrate by UGT enzymes.

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Flow Diagram for Preparation of the UGT-Glo Reagents.

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Panel A is data generated from a panel of UGT isoforms using our UGT Multienzyme Substrate. Panel B is data on the same panel using our UGT1A4 Substrate.

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The UGT-Glo™ Assay can measure the activity of known UGT substrates and inhibitors with various isoforms.

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Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.