A Bright New Way to Measure UGT Activity
Speed: The luminescent format eliminates the need for time-consuming analyses such as HPLC and LC/MS.
Simplified Method: The simple "add and read" protocol makes the assay amenable to high-throughput screening in multiwell plates.
Sensitive: Requires less enzyme and allows you to scale down reaction volumes which saves on reagent costs.
No Fluorescence Interference: The assay system eliminates fluorescence interference by using luminescence to monitor enzyme activity.
Low False-Positive Rate: Use of a proprietary stabilized firefly luciferase (Ultra-Glo™ Recombinant Luciferase) and a proprietary luciferase buffer formulation minimizes the incidence of false positives due to luciferase inhibition.
Conversion of UGT Multienzyme Substrate by UGT enzymes.
Flow Diagram for Preparation of the UGT-Glo Reagents.
Panel A is data generated from a panel of UGT isoforms using our UGT Multienzyme Substrate. Panel B is data on the same panel using our UGT1A4 Substrate.
The UGT-Glo™ Assay can measure the activity of known UGT substrates and inhibitors with various isoforms.